Protein Microarrays and Two-Hybrid Screening Protein microarrays have also been used to study interactions between proteins.
These are large-scale adaptations of the basic two-hybrid screen .
The premise behind the two-hybrid screen is that most eukaryotic transcription factors have modular activating and binding domains that can still activate transcription even when split into two separate fragments, as long as the fragments are brought within close proximity to each other.
If the two proteins of interest bind each other, then the BD and AD will also come together and activate a reporter gene that signals interaction of the two hybrid proteins.
If humans were to artificially intervene and fertilize the egg of a bald eagle with the sperm of an African fish eagle and a chick did hatch, that offspring, called a hybrid (a cross between two species), would probably be infertile: unable to successfully reproduce after it reached maturity.
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