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In plants, carbon dioxide (CO2) enters the leaves through stomata, where it diffuses over short distances through intercellular spaces until it reaches the mesophyllcells.
Once in the mesophyll cells, CO2 diffuses into the stroma of the chloroplast, the site of light-independent reactions of photosynthesis.
These reactions actually have several names associated with them.
Other names for light-independent reactions include the Calvin cycle, the Calvin-Benson cycle, and dark reactions.
The most outdated name is dark reactions, which can be misleading because it implies incorrectly that the reaction only occurs at night or is independent of light, which is why most scientists and instructors no longer use it.
The light-independent reactions of the Calvin cycle can be organized into three basic stages: fixation, reduction, and regeneration.
Stage 1: Fixation
In the stroma, in addition to CO2,two other components are present to initiate the light-independent reactions: an enzyme called ribulose bisphosphate carboxylase (RuBisCO) and three molecules of ribulose bisphosphate (RuBP).
RuBP has five atoms of carbon, flanked by two phosphates.
RuBisCO catalyzes a reaction between CO2 and RuBP.
For each CO2 molecule that reacts with one RuBP, two molecules of 3-phosphoglyceric acid (3-PGA) form.
3-PGA has three carbons and one phosphate.
Each turn of the cycle involves only one RuBP and one carbon dioxide and forms two molecules of 3-PGA.
The number of carbon atoms remains the same, as the atoms move to form new bonds during the reactions (3 atoms from 3CO2 + 15 atoms from 3RuBP = 18 atoms in 3 atoms of 3-PGA).
This process is called carbon fixation because CO2 is "fixed" from an inorganic form into organic molecules.
Stage 2: Reduction
ATP and NADPH are used to convert the six molecules of 3-PGA into six molecules of a chemical called glyceraldehyde 3-phosphate (G3P).
This is a reduction reaction because it involves the gain of electrons by 3-PGA.
Recall that a reduction is the gain of an electron by an atom or molecule.
Six molecules of both ATP and NADPH are used.
For ATP, energy is released with the loss of the terminal phosphate atom, converting it to ADP; for NADPH, both energy and a hydrogen atom are lost, converting it into NADP+.
Both of these molecules return to the nearby light-dependent reactions to be reused and reenergized.
Stage 3: Regeneration
At this point, only one of the G3P molecules leaves the Calvin cycle and is sent to the cytoplasm to contribute to the formation of other compounds needed by the plant.
Because the G3P exported from the chloroplast has three carbon atoms, it takes three "turns" of the Calvin cycle to fix enough net carbon to export one G3P.
But each turn makes two G3Ps, thus three turns make six G3Ps.
One is exported while the remaining five G3P molecules remain in the cycle and are used to regenerate RuBP, which enables the system to prepare for more CO2 to be fixed.
Three more molecules of ATP are used in these regeneration reactions.