To identify the lowest concentration required for a given antibiotic to inhibit bacterial growth, an identical amount of bacteria is introduced into wells of liquid media containing progressively lower concentrations of the drug. (Here, the dilution series of the drug is set up from left to right: for example, well E1 might contain 100 units of drug; E2, 50 units; E3, 25 units; E4, 12.5 units; etc.). Because bacterial growth made the media in well E5 cloudy and the media in well E4 is indistinguishable from clear media, this indicates that the minimum inhibitory concentration is between the drug concentrations in wells E4 and E5. (Image courtesy of Microrao, Dept. of Microbiology, JJMMC, Davangere).
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